1st time playing-none

2nd time-final boss is slightly different (can shoot pea pods)

3rd time-none

4th time-a boss based on butters? (Butter Mouth)

5th time-none

6th time-a secret fight involving text about decimals with byte value

7th time-none

8th-24 times-random easter eggs ([these stack]8th,dev room, 9th, bongos in dev room, 10th, Lunar is in the dev room, 11th, Pea is in dev room, 12th, butter mouth is in room, 13, Glitchface's face is invisible during the finale boss)

25th time-hard mode

26th time-secret Zero boss accessed after the final boss